Metabolic Turnover Studies to Quantify Energy Uptake by Thermogenic Adipose Tissues of Mice


The uptake of glucose, non-esterified fatty acids, and triglycerides into brown adipose tissue is an important determinant of systemic energy metabolism, which can be studied by metabolic turnover studies using radioactive tracers in vivo. Here, we address the uptake of glucose and lipid tracers into metabolically active organs with a focus on thermogenically activated adipose tissues. Uptake by beige and brown adipocytes is highly dependent on conditions such as ambient temperature, but also varies between fasted compared to postprandial states. Accordingly, we provide methodological insights how to quantify glucose and lipid disposal under multiple physiological and environmental conditions.

Bibliographical data

Original languageEnglish
Title of host publicationBrown Adipose Tissue : Methods and Protocols
REQUIRED books only: Number of pages12
Place of PublicationNew York, NY
Publication date2022
ISBN (Print)978-1-0716-2086-1, 978-1-0716-2089-2
ISBN (Electronic)978-1-0716-2087-8
Publication statusPublished - 2022

Comment Deanary

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PubMed 35167093