Dendritic assembly of heteromeric gamma-aminobutyric acid type B receptor subunits in hippocampal neurons.

Standard

Dendritic assembly of heteromeric gamma-aminobutyric acid type B receptor subunits in hippocampal neurons. / Ramírez, Omar A; Vidal, René L; Tello, Judith A; Vargas, Karina J; Kindler, Stefan; Härtel, Steffen; Couve, Andrés.

in: J BIOL CHEM, Jahrgang 284, Nr. 19, 19, 2009, S. 13077-13085.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsätzeForschungBegutachtung

Harvard

Ramírez, OA, Vidal, RL, Tello, JA, Vargas, KJ, Kindler, S, Härtel, S & Couve, A 2009, 'Dendritic assembly of heteromeric gamma-aminobutyric acid type B receptor subunits in hippocampal neurons.', J BIOL CHEM, Jg. 284, Nr. 19, 19, S. 13077-13085.

APA

Ramírez, O. A., Vidal, R. L., Tello, J. A., Vargas, K. J., Kindler, S., Härtel, S., & Couve, A. (2009). Dendritic assembly of heteromeric gamma-aminobutyric acid type B receptor subunits in hippocampal neurons. J BIOL CHEM, 284(19), 13077-13085. [19].

Vancouver

Ramírez OA, Vidal RL, Tello JA, Vargas KJ, Kindler S, Härtel S et al. Dendritic assembly of heteromeric gamma-aminobutyric acid type B receptor subunits in hippocampal neurons. J BIOL CHEM. 2009;284(19):13077-13085. 19.

Bibtex

@article{2a0b3fc986704915bba0ecd4762aed3f,
title = "Dendritic assembly of heteromeric gamma-aminobutyric acid type B receptor subunits in hippocampal neurons.",
abstract = "Understanding the mechanisms that control synaptic efficacy through the availability of neurotransmitter receptors depends on uncovering their specific intracellular trafficking routes. gamma-Aminobutyric acid type B (GABA(B)) receptors (GABA(B)Rs) are obligatory heteromers present at dendritic excitatory and inhibitory postsynaptic sites. It is unknown whether synthesis and assembly of GABA(B)Rs occur in the somatic endoplasmic reticulum (ER) followed by vesicular transport to dendrites or whether somatic synthesis is followed by independent transport of the subunits for assembly and ER export throughout the somatodendritic compartment. To discriminate between these possibilities we studied the association of GABA(B)R subunits in dendrites of hippocampal neurons combining live fluorescence microscopy, biochemistry, quantitative colocalization, and bimolecular fluorescent complementation. We demonstrate that GABA(B)R subunits are segregated and differentially mobile in dendritic intracellular compartments and that a high proportion of non-associated intracellular subunits exist in the brain. Assembled heteromers are preferentially located at the plasma membrane, but blockade of ER exit results in their intracellular accumulation in the cell body and dendrites. We propose that GABA(B)R subunits assemble in the ER and are exported from the ER throughout the neuron prior to insertion at the plasma membrane. Our results are consistent with a bulk flow of segregated subunits through the ER and rule out a post-Golgi vesicular transport of preassembled GABA(B)Rs.",
author = "Ram{\'i}rez, {Omar A} and Vidal, {Ren{\'e} L} and Tello, {Judith A} and Vargas, {Karina J} and Stefan Kindler and Steffen H{\"a}rtel and Andr{\'e}s Couve",
year = "2009",
language = "Deutsch",
volume = "284",
pages = "13077--13085",
journal = "J BIOL CHEM",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "19",

}

RIS

TY - JOUR

T1 - Dendritic assembly of heteromeric gamma-aminobutyric acid type B receptor subunits in hippocampal neurons.

AU - Ramírez, Omar A

AU - Vidal, René L

AU - Tello, Judith A

AU - Vargas, Karina J

AU - Kindler, Stefan

AU - Härtel, Steffen

AU - Couve, Andrés

PY - 2009

Y1 - 2009

N2 - Understanding the mechanisms that control synaptic efficacy through the availability of neurotransmitter receptors depends on uncovering their specific intracellular trafficking routes. gamma-Aminobutyric acid type B (GABA(B)) receptors (GABA(B)Rs) are obligatory heteromers present at dendritic excitatory and inhibitory postsynaptic sites. It is unknown whether synthesis and assembly of GABA(B)Rs occur in the somatic endoplasmic reticulum (ER) followed by vesicular transport to dendrites or whether somatic synthesis is followed by independent transport of the subunits for assembly and ER export throughout the somatodendritic compartment. To discriminate between these possibilities we studied the association of GABA(B)R subunits in dendrites of hippocampal neurons combining live fluorescence microscopy, biochemistry, quantitative colocalization, and bimolecular fluorescent complementation. We demonstrate that GABA(B)R subunits are segregated and differentially mobile in dendritic intracellular compartments and that a high proportion of non-associated intracellular subunits exist in the brain. Assembled heteromers are preferentially located at the plasma membrane, but blockade of ER exit results in their intracellular accumulation in the cell body and dendrites. We propose that GABA(B)R subunits assemble in the ER and are exported from the ER throughout the neuron prior to insertion at the plasma membrane. Our results are consistent with a bulk flow of segregated subunits through the ER and rule out a post-Golgi vesicular transport of preassembled GABA(B)Rs.

AB - Understanding the mechanisms that control synaptic efficacy through the availability of neurotransmitter receptors depends on uncovering their specific intracellular trafficking routes. gamma-Aminobutyric acid type B (GABA(B)) receptors (GABA(B)Rs) are obligatory heteromers present at dendritic excitatory and inhibitory postsynaptic sites. It is unknown whether synthesis and assembly of GABA(B)Rs occur in the somatic endoplasmic reticulum (ER) followed by vesicular transport to dendrites or whether somatic synthesis is followed by independent transport of the subunits for assembly and ER export throughout the somatodendritic compartment. To discriminate between these possibilities we studied the association of GABA(B)R subunits in dendrites of hippocampal neurons combining live fluorescence microscopy, biochemistry, quantitative colocalization, and bimolecular fluorescent complementation. We demonstrate that GABA(B)R subunits are segregated and differentially mobile in dendritic intracellular compartments and that a high proportion of non-associated intracellular subunits exist in the brain. Assembled heteromers are preferentially located at the plasma membrane, but blockade of ER exit results in their intracellular accumulation in the cell body and dendrites. We propose that GABA(B)R subunits assemble in the ER and are exported from the ER throughout the neuron prior to insertion at the plasma membrane. Our results are consistent with a bulk flow of segregated subunits through the ER and rule out a post-Golgi vesicular transport of preassembled GABA(B)Rs.

M3 - SCORING: Zeitschriftenaufsätze

VL - 284

SP - 13077

EP - 13085

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 19

M1 - 19

ER -