Alterations in Rev-ERBα/BMAL1 ratio and glycated hemoglobin in rotating shift workers: the EuRhythDia study

  • Stefano Rizza
  • Alessio Luzi
  • Maria Mavilio
  • Marta Ballanti
  • Arianna Massimi
  • Ottavia Porzio
  • Andrea Magrini
  • Juliane Hannemann
  • Rossella Menghini
  • Michael Lehrke
  • Bart Staels
  • Peter J Grant
  • Rainer H Böger
  • Nikolaus Marx
  • Massimo Federici


OBJECTIVE: To detect premature gluco-metabolic defects among night shift workers with disturbances in circadian rhythms.

DESIGN AND METHODS: We performed a hypothesis-generating, cross-sectional analysis of anthropometric, metabolic, lipid, and inflammation parameters, comparing active (a-NSW, n = 111) and former (f-NSW, n = 98) rotating night shift workers with diurnal workers (controls, n = 69). All participants were hospital nurses. We also evaluated the Pittsburgh Sleep Quality Index (PSQI) and assessed expression of transcription factors REV-ERBα and BMAL1 in peripheral blood mononuclear cells (PBMCs), as indicators of the molecular clock.

RESULTS: Both a-NSW and f-NSW participants had significantly higher glycated hemoglobin (HbA1c) and white blood cell counts (WBC) (p < 0.001 for both), PSQI global score (p = 0.001) and diastolic blood pressure levels (p = 0.024) compared with controls. Expression of REV-ERBα/BMAL1 RNA in PBMC was significantly higher in a-NSW (p = 0.05) than in f-NSW or control participants. Multivariate regression analysis showed that working status and PSQI were independent determinants of higher HbA1c levels (p < 0.001).

CONCLUSIONS: We demonstrated that young, healthy night shift workers show subclinical abnormalities in HbA1c and changes in peripheral clock gene expression.

Bibliografische Daten

StatusVeröffentlicht - 08.2021
PubMed 33788000